Hepatoprotective Effects of Nasturtium Officinale and Allium Polyanthum Extracts on CCL4- Induced Hepatic Damage In Albino Rats
https://doi.org/10.24017/science.2021.2.12
Abstract views: 851 / PDF downloads: 581Abstract
The aim: This study was designed to explore the potential hepatoprotective effects of Nasturtium officinale and Allium polyanthum plant extracts against CCl4-induced hepatic injury in rat model.
Methods: Thirty healthy male albino rats were equally divided into 5 groups: Normal control (NC), Positive control (Pos-C), Nasturtium officinale extract (NOE), Allium polyanthum extract (APE), and NOE + APE. The animals were fed with a standard diet and distilled water ad libitum. Except for NC, all other groups were exposed to CCl4 at every 72 hours for 3 weeks to induce hepatic damage. Corresponding treatments were established to the respective groups during study period. Aspartate aminotransferase (AST), Alanine aminotransferase (ALT), Alkaline phosphatase (ALP), Total serum bilirubin (TSB), Total protein (TP), Albumin (ALB) and malondialdehyde (MDA) were measured in serum at the end of the study. Histopathological analysis of the liver sections was also determined.
Results: CCl4-treated rats were showed significant increase (p < 0.05) in the levels of serum enzyme activities (AST, ALT, & ALP), TSB and MDA; reflecting the hepatocellular damage caused by CCl4 compared with NC rats, whereas the levels of TP and ALB were decreased. Furthermore, the histological examination of liver tissues showed that CCl4 triggered several histological variations in the liver tissues. Treatments of NOE and APE along with CCl4 for 3 weeks significantly (P<0.05) improved the CCl4-induced changes in serum biochemical parameters and liver histology as compared to Pos-C rats indicating the protection of hepatic cells.
Conclusion: Treatments of NOE and APE either in separate or in combination have shown significant improvement in CCl4-induced hepatopathy in experimental rats as evidenced by observed results in serum and hepatic histopathological examinations
Keywords:
Carbon tetrachloride, CCl4 Hepatotoxicity, Hepatic damage, Rat, Nasturtium officinale and Allium polyanthum.
References
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https://doi.org/10.31254/phyto.2014.3207
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https://doi.org/10.3892/etm.2012.840
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https://doi.org/10.1016/j.jep.2010.04.019
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https://doi.org/10.1080/13880200590963952
https://doi.org/10.21608/ejhm.2019.43804
[2] N. Aghel, I. Rashidi, and A. Mombeini, "Hepatoprotective activity of Capparis spinosa root bark against CCl4 induced hepatic damage in mice," 2007.
[3] B. El-Aarag, A. Khairy, S. A. Khalifa, and H. R. El-Seedi, "Protective Effects of Flavone from Tamarix aphylla against CCl4-Induced Liver Injury in Mice Mediated by Suppression of Oxidative Stress, Apoptosis and Angiogenesis," International journal of molecular sciences, vol. 20, no. 20, pp. 5215, 2019.
https://doi.org/10.3390/ijms20205215
[4] R. K. Giri, A. Bose, and S. K. Mishra, "Hepatoprotective activity of Tagetes erecta against carbon tetrachloride-induced hepatic damage in rats," Acta Poloniae Pharmaceutica n Drug Research, vol. 68, no. 6, pp. 999-1003, 2011.
[5] M. G. Palanivel, B. Rajkapoor, R. S. Kumar, J. W. Einstein, E. P. KUMAR, M. R. KUMAR, K. KAVITHA, M. P. KUMAR, and B. JAYAKAR, "Hepatoprotective and antioxidant effect of Pisonia aculeata L. against CCl4-induced hepatic damage in rats," Scientia pharmaceutica, vol. 76, no. 2, pp. 203-216, 2008.
https://doi.org/10.3797/scipharm.0803-16
[6] K. Hegde, and A. B. Joshi, "Hepatoprotective and antioxidant effect of Carissa spinarum root extract against CCI4-and paracetamol-induced hepatic damage in rats," ||| Bangladesh Journal of Pharmacology, vol. 5, no. 1, pp. 73-76, 2010.
https://doi.org/10.3329/bjp.v5i1.5681
[7] T. Jayakumar, E. Ramesh, and P. Geraldine, "Antioxidant activity of the oyster mushroom, Pleurotus ostreatus, on CCl4-induced liver injury in rats," Food and chemical toxicology, vol. 44, no. 12, pp. 1989-1996, 2006.
https://doi.org/10.1016/j.fct.2006.06.025
[8] A. Srivastava, and T. Shivanandappa, "Hepatoprotective effect of the root extract of Decalepis hamiltonii against carbon tetrachloride-induced oxidative stress in rats," Food chemistry, vol. 118, no. 2, pp. 411-417, 2010.
https://doi.org/10.1016/j.foodchem.2009.05.014
[9] O. A. Adesanoye, and E. O. Farombi, "Hepatoprotective effects of Vernonia amygdalina (astereaceae) in rats treated with carbon tetrachloride," Experimental and Toxicologic Pathology, vol. 62, no. 2, pp. 197-206, 2010.
https://doi.org/10.1016/j.etp.2009.05.008
[10] A. T. Williams, and R. F. Burk, "Carbon tetrachloride hepatotoxicity: an example of free radical-mediated injury." pp. 279-284.
https://doi.org/10.1055/s-2008-1040483
[11] M. Ahn, J. S. Park, S. Chae, S. Kim, C. Moon, J. W. Hyun, and T. Shin, "Hepatoprotective effects of Lycium chinense Miller fruit and its constituent betaine in CCl4-induced hepatic damage in rats," Acta histochemica, vol. 116, no. 6, pp. 1104-1112, 2014.
https://doi.org/10.1016/j.acthis.2014.05.004
[12] C.-F. Tsai, Y.-W. Hsu, W.-K. Chen, W.-H. Chang, C.-C. Yen, Y.-C. Ho, and F.-J. Lu, "Hepatoprotective effect of electrolyzed reduced water against carbon tetrachloride-induced liver damage in mice," Food and Chemical Toxicology, vol. 47, no. 8, pp. 2031-2036, 2009.
https://doi.org/10.1016/j.fct.2009.05.021
[13] A. Laouar, F. Klibet, E. Bourogaa, A. Benamara, A. Boumendjel, A. Chefrour, and M. Messarah, "Potential antioxidant properties and hepatoprotective effects of Juniperus phoenicea berries against CCl4 induced hepatic damage in rats," Asian Pacific journal of tropical medicine, vol. 10, no. 3, pp. 263-269, 2017.
https://doi.org/10.1016/j.apjtm.2017.03.005
[14] J.-S. Deng, Y.-C. Chang, C.-L. Wen, J.-C. Liao, W.-C. Hou, S. Amagaya, S.-S. Huang, and G.-J. Huang, "Hepatoprotective effect of the ethanol extract of Vitis thunbergii on carbon tetrachloride-induced acute hepatotoxicity in rats through antioxidative activities," Journal of Ethnopharmacology, vol. 142, no. 3, pp. 795-803, 2012.
https://doi.org/10.1016/j.jep.2012.06.003
[15] R. H. Hussein, and Z. H. Ibrahim, "Influence of Omega-3 and Green Tea Extract on Alcohol-Induced Liver Injury in Rats," Kurdistan Journal of Applied Research, pp. 107-116, 2018.
https://doi.org/10.24017/science.2018.2.18
[16] Z. A. Malik, and P. L. Sharma, "An ethanolic extract from licorice (glycyrrhiza glabra) exhibits anti-obesity effects by decreasing dietary fat absorption in a high fat diet-induced obesity rat model," International Journal of Pharmaceutical Sciences and Research, vol. 2, no. 11, pp. 3010, 2011.
[17] D. Kasote, Y. Badhe, A. Zanwar, M. Hegde, and K. Deshmukh, "Hepatoprotective potential of ether insoluble phenolic components of n-butanol fraction (EPC-BF) of flaxseed against CCl4-induced liver damage in rats," Journal of pharmacy & bioallied sciences, vol. 4, no. 3, pp. 231, 2012.
https://doi.org/10.4103/0975-7406.99064
[18] M. Shahjahan, K. Sabitha, M. Jainu, and C. S. Devi, "Effect of Solanum trilobatum against carbon tetra chloride induced hepatic damage in albino rats," Indian Journal of Medical Research, vol. 120, pp. 194-198, 2004.
[19] G. Robertson, I. Leclercq, and G. C. Farrell, "II. Cytochrome P-450 enzymes and oxidative stress," American Journal of Physiology-Gastrointestinal and Liver Physiology, vol. 281, no. 5, pp. G1135-G1139, 2001.
https://doi.org/10.1152/ajpgi.2001.281.5.G1135
[20] K. Raju, G. Anbuganapathi, V. Gokulakrishnan, B. Rajkapoor, B. Jayakar, and S. Manian, "Effect of Dried Fruits of Solanum nigrum L INN against CCl4-Induced Hepatic Damage in Rats," Biological and Pharmaceutical Bulletin, vol. 26, no. 11, pp. 1618-1619, 2003.
https://doi.org/10.1248/bpb.26.1618
[21] L. Ranawat, J. Bhatt, and J. Patel, "Hepatoprotective activity of ethanolic extracts of bark of Zanthoxylum armatum DC in CCl4 induced hepatic damage in rats," Journal of ethnopharmacology, vol. 127, no. 3, pp. 777-780, 2010.
https://doi.org/10.1016/j.jep.2009.10.019
[22] O. Adewale, A. Adekeye, C. Akintayo, A. Onikanni, and S. Sabiu, "Carbon tetrachloride (CCl4)-induced hepatic damage in experimental Sprague Dawley rats: Antioxidant potential of Xylopia aethiopica," Carbon tetrachloride (CCl4)-induced hepatic damage in experimental Sprague Dawley rats: Antioxidant potential of Xylopia aethiopica, vol. 3, no. 2, pp. 1-6, 2014.
https://doi.org/10.31254/phyto.2014.3207
[23] M. H. Abdulrazzaq, E. J. Khadeem, and S. S. Al-Muhammadi, "Hepatoprotective effect of Echinops tenuisectus (Compositae) on CCl4 induced hepatic damage in rats," Iraqi J Pharm Sci, vol. 17, no. 1, pp. 16-24, 2008.
https://doi.org/10.31351/vol17iss1pp16-24
[24] M. G. Sturgill, and G. H. Lambert, "Xenobiotic-induced hepatotoxicity: mechanisms of liver injury and methods of monitoring hepatic function," Clinical chemistry, vol. 43, no. 8, pp. 1512-1526, 1997.
https://doi.org/10.1093/clinchem/43.8.1512
[25] X. Zhao, "Hawk tea (Litsea coreana Levl. var. lanuginose) attenuates CCl4?induced hepatic damage in Sprague?Dawley rats," Experimental and Therapeutic Medicine, vol. 5, no. 2, pp. 555-560, 2013.
https://doi.org/10.3892/etm.2012.840
[26] R. A. R. Elgawish, H. G. A. Rahman, and H. M. Abdelrazek, "Green tea extract attenuates CCl4-induced hepatic injury in male hamsters via inhibition of lipid peroxidation and p53-mediated apoptosis," Toxicology Reports, vol. 2, pp. 1149- 1156, 2015.
https://doi.org/10.1016/j.toxrep.2015.08.001
[27] V. Jaishree, and S. Badami, "Antioxidant and hepatoprotective effect of swertiamarin from Enicostemma axillare against D-galactosamine induced acute liver damage in rats," Journal of ethnopharmacology, vol. 130, no. 1, pp. 103-106, 2010.
https://doi.org/10.1016/j.jep.2010.04.019
[28] K. Mani Senthilkumar, B. Rajkapoor, and S. Kavimani, "Protective effect of enicostemma littorale. against CCl4-induced hepatic damage in rats," Pharmaceutical biology, vol. 43, no. 5, pp. 485-487, 2005.
https://doi.org/10.1080/13880200590963952
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[1]
S. R. K.Rostam, Z. H. Ibrahim, R. R. Mala, and S. Q. Abdul, “Hepatoprotective Effects of Nasturtium Officinale and Allium Polyanthum Extracts on CCL4- Induced Hepatic Damage In Albino Rats”, KJAR, vol. 6, no. 2, pp. 127–135, Jan. 2022, doi: 10.24017/science.2021.2.12.
Published
24-01-2022
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Pure and Applied Science
