Antibacterial Effect of Fixed and Volatile Oils against Gram-positive and Gram-negative Bacteria

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  • Tara Faiq M. Salih Medical Laboratory Department, Technical College of Health, Sulaimani Polytechnic University, Sulaimani, Iraq
  • Sakar Ahmed Abdullah Medical Laboratory Department, Technical College of Health, Sulaimani Polytechnic University, Sulaimani, Iraq
  • Salar Ibrahim Ali Nursing department, College of Health, Sulaimani Polytechnic University, Sulaimani, Iraq
  • Daryan Kamal Khder Nursing department, College of Health, Sulaimani Polytechnic University, Sulaimani, Iraq


Antibiotic resistance phenomena among pathogenic bacteria considered as a major health problem ?and associated with increased mortality or long-term hospitalization, which lead to open a new era by using ?plant and herbal extracts as an alternative source of various chemotherapeutic drugs, also to increase antibiotic efficiency by combining with plant extract for obtaining a powerful and broad ?spectrum action. The current investigation aims to investigate antibacterial actions of fixed oils of (Olea europaea L., ?Ricinus communis L. and Linum usitatissimum) and volatile oil of (Nigella sativa, Curcuma longa L and ?Zingiber officinale) against both Staphylococcus aureus strain (6734151) and Escherichia coli ?strain (5344572). This study conducted on antibacterial effect of six ?different extracted oils from medical herbs. The findings revealed that the oil extracts have different ?antibacterial activities with efficacy. Bacterial inhibition zone was ?detected by using ?disk diffusion method. Furthermore, volatile oil of N. sativa ?showed a great inhibitory action ?against ?resistant S. aureus, which was (27.7± 1.2 mm). ?The antimicrobial effects of other fixed and volatile oils ?against S. aureu, the inhibition zone was (10 ± 1.0 mm) for (Zingiber officinale), ? (9 ± 1.0 mm) for Ricinus communis L., (7.7 ???± 0.6 mm) for Olea europea L., (7.3 ± 0.6 mm) for Linum usitatissimum and for Curcuma ?longa L. was (6.7 ± 0.6mm). Moreover, antimicrobial effect of N. sativa against ? E. coli was more ??active in comparison with other oils, while other oils showed a slight antibacterial effect?. In conclusion, volatile oil of N. sativa reveals great antibacterial activities in comparison with other extracted oils. 



Black seed, antibacterial activity, fixed oil and volatile oil.


[1] M. Gostynski et al., "Analysis of the relationship between total cholesterol, age, body mass index among males and females in the WHO MONICA Project," Int. J. Obes., vol. 28, no. 8, pp. 1082-1090, Aug. 2004.
[2] "Comparison of Antibacterial Activities of Selected Species of Zingiberaceae Family and Some Synthetic Compounds."
[3] A. Bugno, M. A. Nicoletti, A. A. B. Almodóvar, T. C. Pereira, and M. T. Auricchio, "Antimicrobial efficacy of Curcuma zedoaria extract as assessed by linear regression compared with commercial mouthrinses," Brazilian J. Microbiol., 2007.
[4] B. Doro, "Studies of the antimicrobial activity of Black Seed Oil from Nigella Sativaon Staphylococcus aureus and Escherichia coli."
[5] Q. Liu, X. Meng, Y. Li, C. N. Zhao, G. Y. Tang, and H. Bin Li, "Antibacterial and antifungal activities of spices," International Journal of Molecular Sciences. 2017.
[6] M. Brenes, E. Medina, C. Romero, and A. De Castro, "Antimicrobial activity of olive oil," Agro Food Ind. Hi. Tech., vol. 18, no. 4, pp. 6-8, 2007.
[7] S. N. Malik, "Antibacterial activity of olive (Olea europaea)leaves and arugula (Eruca sativa) seeds extract," Int. J. Pharmacogn. Phytochem. Res., vol. 7, no. 2, pp. 307-310, 2015.
[8] E. Medina, A. De Castro, C. Romero, E. Ramírez, and M. Brenes, "Effect of antimicrobial compounds from olive products on microorganisms related to health , food and agriculture," Sci. Technol. Educ., pp. 1087-1094, 2013.
[9] A. P. Pereira et al., "Phenolic Compounds and Antimicrobial Activity of Olive (," Molecules, pp. 1153-1162, 2007.
[10] A. . Momoh, M. K. Oladunmoye, and T. T. Adebolu, "Evaluation of the antimicrobial and phytochemical properties of oil from castor seeds ( Ricinus communis Linn)," Bull. Environ. Pharmacol. Life Sci., vol. 1, no. 10, pp. 21-27, 2012.
[11] E. Medicines Agency, "Assessment report on Ricinus communis L., oleum," vol. 44, no. July, 2015.
[12] M. M. SALLES, V. de C. OLIVEIRA, R. F. SOUZA, C. H. L. SILVA, and H. de F. O. PARANHOS, "Antimicrobial action of sodium hypochlorite and castor oil solutions for denture cleaning - in vitro evaluation," Braz. Oral Res., vol. 29, no. 1, pp. 1-6, 2015.
[13] M. Hassan Houshdar Tehrani, R. Batal, M. Kamalinejad, and A. Mahbubi, "Extraction and purification of flaxseed proteins and studying their antibacterial activities," J. Plant Sci., vol. 2, no. 1, pp. 70-76, 2014.

[14] K. K. Singh, D. Mridula, J. Rehal, and P. Barnwal, "Flaxseed: A potential source of food, feed and fiber," Crit. Rev. Food Sci. Nutr., vol. 51, no. 3, pp. 210-222, 2011.
[15] Z. A. H. Al- Ebady, "Evaluation of the inhibitory effect of a number of medicinal plants fixed oils on a group of pathogenic bacteria invitro," Al-Qadisiyah J. Vet. Med. Sci., vol. 10, no. 1, p. 36, 2019.
[16] H. J. F. Al-Mathkhury, A. S. Al-Dhamin, and K. L. Al-Taie, "Antibacterial and Antibiofilm Activity of Flaxseed Oil," Antibact. Antibiofilm Act. Flaxseed Oil, vol. 57, no. 2B, pp. 1086-1095, 2016.
[17] A. A. H. S. AL Janabi, "Archives of Medical Biotechnology Inhibitory Action of Essential Oils on Quorum Sensing Activity of Pseudomonas aeruginosa by Effecting on Pyocyanin Production," Arch Med Biotechnol, vol. 1, no. 2, pp. 9-12, 2018.
[18] G. Kaithwas, A. Mukerjee, P. Kumar, and D. K. Majumdar, "Linum usitatissimum (linseed/flaxseed) fixed oil: Antimicrobial activity and efficacy in bovine mastitis," Inflammopharmacology, vol. 19, no. 1, pp. 45-52, 2011.
[19] K. M. Kumar, G. Asish, M. Sabu, and I. Balachandran, "Significance of gingers (Zingiberaceae) in Indian System of Medicine - Ayurveda: An overview," Anc. Sci. Life, vol. 32, no. 4, p. 253, 2014.
[20] S. Anjusha and A. Gangaprasad, "Phytochemical and Antibacterial Analysis of Two Important Curcuma species, Curcuma aromatica Salisb. and Curcuma xanthorrhiza Roxb.(Zingiberaceae)," J. Pharmacogn. …, vol. 3, no. 3, pp. 50-53, 2014.
[21] H. Wohlmuth, "Phytochemistry and pharmacology of plants from the ginger family, Zingiberaceae," Res. Sch. Publ. South. Cross Univ., pp. 1-237, 2008.
[22] M. L. Tsai, C. T. Wu, T. F. Lin, W. C. Lin, Y. C. Huang, and C. H. Yang, "Chemical composition and biological properties of essential oils of two mint species," Trop. J. Pharm. Res., vol. 12, no. 4, pp. 577-582, 2013.
[23] J. Dhanik, N. Arya, and V. Nand, "A {Review} on {Zingiber} officinale," J. Pharmacognpsy Phytochem., vol. 6, no. 3, p. 11, 2017.
[24] A. Akhtar, "Physico-Chemical Characterization and Product Development from Ginger (Zingiber officinalis) Germplasm Available in South Western Region of Bangladesh," IOSR J. Agric. Vet. Sci., vol. 5, no. 6, pp. 53-62, 2014.
[25] J. B. Zadeh and N. M. Kor, "Physiological and pharmaceutical effects of Ginger ( Zingiber officinale Roscoe ) as a valuable medicinal plant Faculty of Animal Science , Shahid Bahonar University , Kerman , Iran Jalal Bayati Zadeh and Nasroallah Moradi Kor," Pelagia Res. Libr. Eur., vol. 4, no. 1, pp. 87-90, 2014.
[26] E. M. Abdallah, "Black Seed ( Nigella sativa ) As Antimicrobial Drug?: A Mini-Review Black," Nov. Approaches Drug Des. Dev., vol. 3, no. 2, pp. 2-6, 2017.
[27] N. A. Hasan, M. Z. Nawahwi, and H. A. Malek, "Antimicrobial activity of nigella sativa seed extract," Sains Malaysiana, vol. 42, no. 2, pp. 143-147, 2013.
[28] G. Meenal and B. K. Mehta, "A comprehensive review eview on secondary metabolites of Nigella sativa L ( Seeds )," vol. 7, no. 12, pp. 16-29, 2017.
[29] S. Muthusami, K. Kandar, and K. Kandar, "Antimicrobial activity of selected plant extracts against plant pathogens," vol. 3, no. 1, pp. 130-132, 2008.
[30] M. A. Yassen, "Study of the antibacterial activity of Nigella sativa ethanol extraction the growth of Staphylococcus aureus in culture media," Int. J. Res. Pharm. Sci., vol. 9, no. SPL1, 2018.
[31] T. O. N. Colonization, I. With, T. Aureus, O. Microbes, and I. N. Skin, S TUDIES ON COLONIZATION AND INFECTION WITH Mikael Alsterholm Department of Dermatology and Venereology Sahlgrenska University Hospital Institute of Clinical Sciences Sahlgrenska Academy at the University of Gothenburg Gothenburg , Sweden 2012. 2012.
[32] C. L. Ventola, "The antibiotic resistance crisis: causes and threats.," P T J., vol. 40, no. 4, pp. 277-83, 2015.
[33] F. A. Saleh, N. El-Darra, K. Raafat, and I. El Ghazzawi, "Phytochemical Analysis of Nigella sativa L. Utilizing GC-MS Exploring its Antimicrobial Effects against Multidrug-Resistant Bacteria," Pharmacogn. J., vol. 10, no. 1, pp. 99-105, 2017.
[34] A. A. Mostafa, A. A. Al-Askar, K. S. Almaary, T. M. Dawoud, E. N. Sholkamy, and M. M. Bakri, "Antimicrobial activity of some plant extracts against bacterial strains causing food poisoning diseases," Saudi J. Biol. Sci., vol. 25, no. 2, pp. 361-366, 2018.
[35] D. Ere, K. Pondei, Q. Inaibo, and L. Orutugu, "conyzoides extracted using different solvents Phytochemicals and Antimicrobial activity of plant parts of Ageratum conyzoides extracted using different solvents," vol. 16, no. March 2015, pp. 16-22, 2014.
[36] P. Sinha, S. Srivastava, N. Mishra, and N. P. Yadav, "New Perspectives on Antiacne Plant Drugs: Contribution to Modern Therapeutics," Biomed Res. Int., vol. 2014, 2014.
[37] B. M, S. BR, and S. DK, "Potential of Herbal Drug and Antibiotic Combination Therapy: A New Approach to Treat Multidrug Resistant Bacteria," Pharm. Anal. Acta, vol. 7, no. 11, 2017.
[38] G. U. Bautista-Trujillo et al., "Performance of culture media for the isolation and identification of Staphylococcus aureus from bovine mastitis," J. Med. Microbiol., vol. 62, no. PART3, pp. 369-376, 2013.
[39] J. Zurita, C. Mejía, and M. Guzmán-Blanco, "Diagnosis and susceptibility testing of methicillin-resistant Staphylococcus aureus in Latin America," Brazilian J. Infect. Dis., vol. 14, pp. 97-106, 2010.
[40] K. S. Themptander, "Detection and characterisation of Vibrio harveyi isolates," Manuscript, Dublin Inst. Technol., vol. 29, no. 6, pp. 997-1003, 2012.
[41] T. Farasat, Z. Bilal, and F. Yunus, "Isolation and biochemical identification of Escherichia coli from wastewater effluents of food and beverage industry," J. Cell Mol. Biol., vol. 10, no. 1, pp. 13-18, 2012.
[42] S. Mirrett and L. B. Reller, "Comparison of direct and standard antimicrobial disk susceptibility testing for bacteria isolated from blood," J. Clin. Microbiol., vol. 10, no. 4, pp. 482-487, 1979.
[43] A. Manoharan, R. Pai, V. Shankar, K. Thomas, and M. K. Lalitha, "Comparison of disc diffusion & E test methods with agar dilution for antimicrobial susceptibility testing of Haemophilus influenzae," Indian J. Med. Res., vol. 117, no. FEB., pp. 81-87, 2003.
[44] B. Abbouni, D. Hadjazi, K. L. Daouadji, F. Z. I. Reffas, and M. L. Benine, "Antibacterial Activity of the Essential Oils of Nigella sativa L. against Pathogens Bacteria," Glob. J. Biotechnol. Biochem., vol. 10, no. 2, pp. 100-105, 2015.
[45] J. Ranjitha and S. Vijiyalakshmi, "Facile Methods for the Extraction of Essential Oil From the Plant Species-a Review," Int. J. Pharm. Sci. Res., vol. 5, no. 4, p. 1107, 2014.
[46] K. A. Khalid, "Effect of NP and foliar spray on growth and chemical compositions of some medicinal Apiaceae plants grow in arid regions in Egypt," J. soil Sci. plant Nutr., vol. 12, no. ahead, pp. 0-0, 2012.
[47] S. Geiselhardt et al., "Egg Laying of Cabbage White Butterfly (Pieris brassicae) on Arabidopsis thaliana Affects Subsequent Performance of the Larvae," PLoS One, vol. 8, no. 3, 2013.
[48] R. Dixit, "Carriage of Methicillin Resistant Staphylococcus aureus (MRSA) among Health Care Workers in Cardiac Unit of a Tertiary Care Hospital," Int. J. Curr. Microbiol. Appl. Sci., vol. 7, no. 03, pp. 2674-2679, 2018.
[49] M. H. Sureshjani, F. T. Yazdi, B. A. Behbahani, and A. Mortazavi, "Antimicrobial Effect of Aqueous, Ethanol, Methanol and Glycerin Extracts of Satureja bachtiarica on Streptococcus pyogenes, Pseudomonas aeruginosa and Staphylococcus epidermidis," Zahedan J. Res. Med. Sci., vol. 16, no. 7, pp. 21-25, 2013.
[50] H. Hosseinzadeh, B. S. Fazly Bazzaz, and M. M. Haghi, "Antibacterial activity of total extracts and essential oil of Nigella sativa L. seeds in mice," Pharmacologyonline, vol. 2, no. January, pp. 429-435, 2007.
[51] M. Hyldgaard, T. Mygind, and R. L. Meyer, "Essential oils in food preservation: Mode of action, synergies, and interactions with food matrix components," Front. Microbiol., vol. 3, no. JAN, pp. 1-24, 2012.
[52] J. Sharifi-Rad et al., Biological activities of essential oils: From plant chemoecology to traditional healing systems, vol. 22, no. 1. 2017.
[53] A. Hannan, S. Saleem, S. Chaudhary, M. Barkaat, and M. Arshad, "ANTI BACTERIAL ACTIVITY OF NIGELLA SATIVA AGAINST CLINICAL ISOLATES OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS Abdul Hannan , Sidrah Saleem , Saadia Chaudhary *, Muhammad Barkaat ," J Ayub Med Coll Abbottabad, vol. 20, no. 3, pp. 72-74, 2008.
[54] K. A. Hammer, C. F. Carson, and T. V Riley, "Antimicrobial activity of essential oils and other plant extracts.," J. Appl. Microbiol., vol. 86, no. 6, pp. 985-90, 1999.
[55] U. Emeka, N. G. Iloegbunam, and A. R. Gbekele-Oluwa, "Natural Products and Aquaculture Development," IOSR J. Pharm. Biol. Sci., vol. 9, no. 2, pp. 70-82, 2014.
[56] M. E. Abd El-Hack, M. Alagawany, M. R. Farag, R. Tiwari, K. Karthik, and K. Dhama, "Nutritional, healthical and therapeutic efficacy of black cumin (Nigella sativa) in animals, poultry and humans," Int. J. Pharmacol., vol. 12, no. 3, pp. 232-248, 2016.
[57] B. Boubakeur, A. Tirtouil, B. Meddah, and H. Khadem, "The evaluation of the effect of synthetic flavonoids on growth of pathogenic and probiotic bacteria," J. Chem. Pharm. Res., vol. 7, no. 10, pp. 228-236, 2015.
[58] Ali Ghasemzadeh, "Flavonoids and phenolic acids: Role and biochemical activity in plants and human," J. Med. Plants Res., vol. 5, no. 31, pp. 6697-6703, 2011.
[59] Analele, "Plant Products As Antimicrobial Agents Ionela Daciana Ciocan * , Ion I. B?ra," pp. 151-156, 2007.
[60] I. Barák and K. Muchová, "The role of lipid domains in bacterial cell processes," Int. J. Mol. Sci., vol. 14, no. 2, pp. 4050-4065, 2013.
[61] Jehan Bakht, "Antimicrobial activities of different solvents extracted samples of Linum usitatissimum by disc diffusion method," African J. Biotechnol., vol. 10, no. 85, pp. 19825-19835, 2012.
[62] C. J. Zheng, J. S. Yoo, T. G. Lee, H. Y. Cho, Y. H. Kim, and W. G. Kim, "Fatty acid synthesis is a target for antibacterial activity of unsaturated fatty acids," FEBS Lett., vol. 579, no. 23, pp. 5157-5162, 2005.
[63] I. Leouifoudi, H. Harnafi, and A. Zyad, "Olive Mill Waste Extracts: Polyphenols Content, Antioxidant, and Antimicrobial Activities," Adv. Pharmacol. Sci., vol. 2015, pp. 1-11, 2015.
[64] H. Zengin and A. H. Baysal, "Antibacterial and antioxidant activity of essential oil terpenes against pathogenic and spoilage-forming bacteria and cell structure-activity relationships evaluated by SEM microscopy," Molecules, vol. 19, no. 11, pp. 17773-17798, 2014.
[65] S. G. Ali, E. K. Bakhiet, and U. M. Abdul-Raouf, "Antibacterial Activities of THYMUS VULGARIS and Rifmpicin on Methicillin Resistant STAPHYLOCOCCUS AUREUS (MRSA)," J. Ecol. Heal. Environ., vol. 1, no. 1, pp. 19-24, 2013.
[66] F. G. C. de Lima, M. A. P. da Silva, B. T. Freitas, J. C. M. Freitas, C. de S. Teixeira, and J. W. A. Bezerra, "Evaluation of antimicrobial activity of the fixed oil of Bowdichia virgilioides Kunth (Fabaceae) seeds," Gaia Sci., vol. 10, no. 4, pp. 681-689, 2018.
[67] L. KOKOSKA, J. HAVLIK, I. VALTEROVA, H. SOVOVA, M. SAJFRTOVA, and I. JANKOVSKA, "Comparison of Chemical Composition and Antibacterial Activity of Nigella sativa Seed Essential Oils Obtained by Different Extraction Methods," J. Food Prot., vol. 71, no. 12, pp. 2475-2480, 2016.


How to Cite

T. Faiq M. Salih, S. Ahmed Abdullah, S. Ibrahim Ali, and D. Kamal Khder, “Antibacterial Effect of Fixed and Volatile Oils against Gram-positive and Gram-negative Bacteria”, KJAR, pp. 74–87, Aug. 2019, doi: 10.24017/science.2019.ICHMS.8.

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