Laboratory diagnosis of H. pylori among dyspeptic patients using Culture and Rapid urease test

Abstract views: 1876 / PDF downloads: 815


  • Hogir Mohammed Shukri Saadi Directorate of Akre education, General directorate of duhok education, Ministry of Education, Duhok Iraq
  • Ali Yahya Saeed Department of Biology, College of Science, University of Duhok, Duhok, Iraq


Globally Helicobacter pylori has been defined is the prime reason of stomach ulcer and gastric cancer. Medical laboratory analysis of H. pylori infection is done by two ways invasive and non- invasive methods. Invasive techniques frequently disapproved by patients because it is inconvenient but still remain reliable methods. Among invasive methods, culture is considered as gold standard method from which other methods are compared. A sum of eighty six persons with average of ages between  18-77 years old with mean 37.58 years ± (forty three males ,  forty three females) who visited to endoscopic center / Azadi Teaching Hospital for endoscopic examination from June to Oct. 2013.From everyone , two antral biopsies, one for urease test, the other for culture were collected. Biographies from each case were taken in a questionnaire form after approval by Research Ethics Committee of the college of medicine / Duhok university. H. pylorus was found in 37.2% and 68% by Culture and Rapid urease test respectively. The biggest value percentage of H. pylori positive cases was detected by rapid urease test while the fewest value percentage was by Culture. Combination of both tests it did not approve as a diagnostic test for detection of this pathogenic bacteria. The study did not detect any statistical correlation on the impact of age factor on H. pylori infection by both methods. In this research appear males were less positive for H. pylori than females by urease test and no such statistical association was noticed count on the sex and Helicobacter pylori pathogenicity via culture. Research never noticed any considerable correlation was found between smoking status and patient's residence with H. pylori positive cases by both methods. This study was performed to estimate the appropriate and better diagnostic tests for diagnosis of H. pylori among various types of samples. Due to the increasing incidence of treatment failure (caused in part by antibiotic resistance), post-treatment testing is recommended to confirm H. pylori eradication. Knowledge of the epidemic and the Routes of transmission of this pathogen are important points to avoid from spreading and may be useful in identifying high-risk populations, especially in areas that have high rates of gastric lymphoma, gastric cancer, and gastric ulcer. The current study concludes (RUT) was superlative than culture for the detection of Helicobacter pylori.


Rapid urease test , Culture, Helicobacter pylori


[1] M. Arshad , M. Akram , U. Shahab, A. Afzal, U. Khan, H. Abdul, and A. M. Mohiuddin, "Helicobacter pylori: An introduction," International Journal of Applied Biology and Pharmaceutical Technology , vol.1, pp. 1337-1351, 2010.
[2] R. M. Abu-Mughesieb , "Risk Factors Associated with Helicobacter pylori Infection in Gaza, Palestine. M. Sc. Thesis The Islamic University-Gaza, Deanship of Graduate Studies, Biological Sciences Master Program, Medical Technology, Facutly of Science," 2007.
[3] V. Gabriel, "Microbiology and Infectious disease,"1997 3rd ed. williams and wilkins U. S. A.
[4] M. Amini, A. Karbasi, and H. Khedmat, "Evaluation of eating habits in dyspeptic patients with or without Helicobacter pylori infection," Journal of Tropical Gastroenterology, vol. 30(3) , pp.142-144, 2009.
[5] A. S. Barik , "Helicobacter pylori Infection in Developing Countries: The Burden for How Long?," Saudi Journal of Gastroenterology,vol. 15(3), pp. 201-207, 2009.
[6] S.A.Margaret,"Transmission of Helicobacter pylori," Postgraduate medical journal,vol.75, pp.198-200, 1999.
[7] C.P. Dooley, H. Cohen , P. L. Fitzgibbons, M. Bauer, M.D. Appleman, G. I. Perez-Perez, and M. J. Blaser, "Prevalence of Helicobacter pylori infection and histologic gastritis in asymptomatic persons," N England Journal Medical ,vol. 1(23), pp. 1562-1566, 1989.
[8] N. R. Ndip, E. A. Malange,T. F. J.Akoachere, G. W. MacKay, K. P. V. Titanji and T. L. Weaver, "Helicobacter pylori antigens in the faeces of asymptomatic children in the Buea and Limbe health districts of Cameroon," A pilot study Tropical Medicine and International Health, vol.9(9), pp.1036-1040, 2004.
[9] J.A. Shepherd, L.C. Williams, P.C. Doherty, M. Hossack, T. Preston, E.L. Kenneth, L.E.K. McColl, and L.T.Weaver, " Comparison of an enzyme immunoassay for the detection of Helicobacter pylori antigens in the faeces with the urea breath test," Archives of Disease in Childhood,vol.83(3),pp.268-270, 2000.
[10] N.F.Tanih,A.M.Clarke, N. Mkwetshana, E. Green, L.M. Ndip, and R.N.Ndip," Helicobacter pylori infection in Africa," Pathology and microbiological diagnosis. African Journal of Biotechnology,vol 7(25),pp.4653-4662, 2008.
[11] D. Callote,"Prevalence and Risk factors for Helicobacter pylori Transmission in this Eastern Cape provence," Application of Immunological , Molecular and Demographic methods. Thesis M.Sc. Department of Biochemistry and Microbiology, University of Fort Hare. 2009
[12] C.Dube,N.F.Tanih,A.M.Clarke, N. Mkwetshana, E. Green and R.N.Ndip,"Helicobacter pylori infection and transmission in Africa: household hygiene and water sources as plausible factors exacerbating spread," African Journal of Biotechnology,vol 8(22),pp.6028-6035, 2009.
[13] F. Ravizi and A. Hanan," Evaluation of different transport and enrichment media for the isolation Helicobacter pylori," Journal of Ayub Medical College, vol 12(3),pp.31-33,(2000).
[14] A.Y.Saeed, and S.M.Pedawi,"Prevalence of Helicobacter pylori Among Dyspeptic Patients," Journal of Zankoy sulaimani, vol 5 (2),pp. 37-42, 2000.
[15] A.Bazargani, A. R. Ekrami, E. Bassiri, and M. S. Firoozi,"Frequency of CagA in Helicobacter Pylori Isolates of Patients with Peptic Ulcer Diseases (PUD) and Nonulcer Dyspepsia (NUD) at Namazi Hospital," Shiraz, Iran ,Govaresh, vol 10(2),pp. 116-119, 2005.
[16] B. C. Y. Wong, W.M.Wong, W.H.Wang, V. S. Y. Tang,J.Young,K.C.Lia,S.T.Yuen,S.YLeung,W.H.C. Hu, C. K. Chan, W.M.Hui, and S. K. Lam, "An evaluation of invasive and non-invasive tests for the diagnosis of Helicobacter pylori infection in Chinese," Alimentary Pharmacology & Therapeutics, vol 15(4),pp. 505-511, 2001.
[17] R. Johaneesssen, K. Bergh, C. Jianu, and P. M. Klevel, "Polymerase chain reaction versus culture diagnosis of Helicobacter pylori infection," Gastroenterology Insights, vol. 5 (1), pp1-6,2013.
[18] I. Alsaimary, M. Al-Saadon, A. Jassim, and S. Hammadi, "Clinical Findings and Prevalence of Helicobacter Pylori in Patients with Gastritis B in Al-Basrah Governorate," Oman Medical Journal, vol.24(3), pp. 208-211, 2009.
[19] A.P.Lage , E. Godfroid, A.Fauconnier, A.Burette, Jean-P. Butzler, A. Bollen and Y.Glupczynski, "Diagnosis of Helicobacter pylori Infection by PCR: Comparison with Other Invasive Techniques and Detection of cag A Gene in Gastric Biopsy Specimens," Journal of clinical microbiology,vol 33 (10) ,pp.2752-2756, 1995.
[20] S. H. Z. R. Rahman, M. G. Azam,M.A. Rahman, M.S.Arfin, M.M.Alam,T.M Bhuiyan, A.Nasim, M. Rahman ,Sh.Nahar and M.S. Hassan ," Non-invasive diagnosis of H. pylori infection: Evaluation of serological tests with and without current infection marker CIM," World Journal of Gastroenterology, vol 14(8),pp.1231-1236, 2008.
[21] N. M. Kaore, V. N. Nagdeo, and V .R. Thombare, "Comparative evaluation of the diagnostic tests for Helicobacter pylori and dietary influence for its Acquisition in Dyspeptic patients: A rural hospital based study in central Indi,". Journal of clinical and diagnostic Research, vol. 6 (4), pp. 636-641, 2012.
[22] S. Shukla, M. Pujani, A. Agarwal, and A. Rohtagi, "Correlation of Serology with Morphological Changes in Gastric Biopsy in Helicobacter Pylori Infection and Evaluation of Immunohistochemistry for H. Pylori Identification," The Saudi Journal of Gastroenterology, vol.18(6), pp. 369-374, 2012.
[23] A.Daniel,K.Endale,M.Yohannes,A.Senait,A.Kiros,A.Waleed,N.Ingrid and W.Torkel,"Comparison of diagnostic methods for detection of Helicobacter pylori infection in different clinical samples of Ethiopian Dyspeptic patients," Austral - Asian Journal of Cancer , vol 6(4),pp.231-237, 2007.
[24] A. V. Thillainayagam, A. S. Arvind, R.S. Cook, I .G. Harrison, S. Tabaqchali and M.J.G. Farthing , "Diagnostic efficiency of an ultrarapid endoscopy room test for Helicobacter pylori,"Gut,vol 32(5),pp.467-469,1991.
[25] A. M. S. Ibrahim, and M. K. Turab, "The effect of propolis on growth inhibition of Helicobacter pylori isolates from peptic ulcer patient," Kufa Journal For Veterinary Medical Sciences, vol.2(1), pp.44-58, 2011
[26] H. I. Baqir, M. A. Assam, A. S. Al-Bana, and H. M. Al-Aubaidi, "Sere-prevalence of Helicobacter pylori infection in unselected adult population in Iraq," International Journal of Global Education,vol. 1(3), pp.22-29, 2002.
[27] M.M.S.U.Islam,S.Nahar,M.N.Sarker,A.S.M.Salimullah, M.A..Rahman, M.Q.Islam A. S. M .A .Raihan, D.S.Ahmed,M. R. Bhuiyan, S. A. Sarker and M.T. Jalal," Efficacy of Different Laboratory Tests to Diagnose Helicobacter pylori Infection," Farid pur Medical College Journal, vol 8(1),pp 11-14, 2013.
[28] M. Sharma, P. Mehta, and P. Vohra, "Comparative Evaluation of Different Diagnostic Techniques Available for Diagnosis of Helicobacter Pylori," International Journal of Scientific and Research Publications,vol.2, pp.1-5, 2012
[29] The EUROGAST study group ,"Epidemiology of risk factors for, Helicobacter pylori infection among3194 Asymptomatic subjects in 17 population,"Gut,vol.34,pp1672-1676,1993.
[30] N.Shamsum,K.Kadiri,H.Enayet,S.A.Shafiqul,B.K.Pardip,T.A.Kaiser and R.Motiur,"Epidemiology of H.pylorand its relation with gastrointestinal disorders, A community -based study in Dhaka,Bangladish,"Journal of Gastroenterology and Histology research,vol.7(5),pp.2710-2717,2018.
[31] C. Dahorea, G. Coman and O. Ailiesei,"Diagnostic value of microscopic exam and urease test in Helicobacter pylori species infections," Analele ?tiin?ifice Ale Universit??ii Alexandru Ioan Cuza din Ia?i,Sectiunea II A : Genetica si Biologie Moleculara,vol 5, 2004.
[32] A.Ibrahim,S.Morais,A.Ferro,N.Lunet,B.Peleteiro,"Sex-difference in the prevalence of Helicobacter pylori infection in pediatric and adult population :Systemic review and meta-analysis of 244 studies,"An Internation Journal of Gastroenterology and Hepatology,vol 49(7),pp.742-749,2017
[33] Y.Tayfun,A.Delik,S.Selen and Y.Oya,"The Prevalence of Helicobacter pylori and Related Factors among University Students in Turkey," Japanese Journal of Infectious and Disease.vol 61,pp.179-183.2008.
[34] B.C.Malcolm," Cigarette smoking and Helicobacterpylori infection,"Postgraduate Medical Journal, vol.69,pp.41-44,1993.


How to Cite

H. Mohammed Shukri Saadi and A. Yahya Saeed, “Laboratory diagnosis of H. pylori among dyspeptic patients using Culture and Rapid urease test”, KJAR, pp. 174–181, Aug. 2019, doi: 10.24017/science.2019.ICHMS.18.

Article Metrics





Pure and Applied Science