Antibacterial Activity of Copper Nanoparticles Fabricate via Malva Sylvesteris Leaf Extract

Osama Ismail Haji Zebari; Samie Yaseen Sharaf Zeebaree; Aemn Yaseen Sharaf Zeebaree; Hardan Ismail Haji Zebari; Hadeel Ridha Abbas

doi:10.24017/science.2019.ICHMS.15

Authors

Osama Ismail Haji Zebari Akre Directorate Education, Duhok General Directorate, Ministry of Education, Duhok, Iraq
Samie Yaseen Sharaf Zeebaree Dept. of Medical lab. Technology, Shekhan Technical College of Health, Duhok Polytechnic University, Duhok, Iraq
Aemn Yaseen Sharaf Zeebaree Dept. of Medical lab. Technology, Shekhan Technical College of Health, Duhok Polytechnic University, Duhok, Iraq
Hardan Ismail Haji Zebari Akre Directorate Education, Duhok General Directorate, Ministry of Education, Duhok, Iraq
Hadeel Ridha Abbas Maternity & Obstetric Department, Sulaimani Technical Institute, Sulaimani Polytechnic University, Sulaimani, Kurdistan Region, Iraq

Abstract

A Green reduction of copper ions Cu2+ has been achieved by one-step process and at room temperature utilizing Malva sylvesteris. The extract of Malva Sylvesteris leaf has been identified using qualitative tests to detect the bioactive compounds such as flavonoids, polyphenols, terpenoids and carbohydrates. Characterization of copper nanoparticles was diagnosed by Ultraviolet–visible spectroscopy that confirms the band characteristic for copper nanoparticles in the range of 200–700 nm and the role of Malva Selvesteris leaf extract biomolecules was confirmed by Fourier transform infrared spectroscopy. The crystal shape of nanoparticles was confirmed by X-ray diffraction (XRD) at average (20.96 nm) and the peaks correspond to the face centered cubic structure of cooper metal. Effective antiseptic activity of copper nanoparticles determined by measurement of inhibition zone showed against representative microorganism of bacteria (Gram-positive: Clostridium Staph.aureus;) and (Gram-negative: Escherichia col; Pseudomonas; Klebsiella

Keywords:

Fabrication, Nanoparticles, Malva sylvesteris , Microbial susceptibility

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