The relaxant effect of Tamsulosin in the vascular reactivity of goat isolated renal artery

Abstract views: 1577 / PDF downloads: 788


  • Aveen Muhsin Asaad Medical Laboratory Technology Department, Koya Technical Institute, Erbil Polytechnic University, Koya, Iraq
  • Ismail Salih Ibraheem Kakey Department of Biology, Faculty of Science and Health, Koya University, Erbil, Iraq


Alpha-blockers including tamsulosin, are medications that relax muscles in the urinary tract to facilitate stone passage into the bladder. This research aimed to investigate the possible action of tamsulosin (1 × 10-3 – 10-8 M), in the vascular reactivity of goat isolated renal artery by using the organ bath and PowerLab data acquisition system. The results of recording and analysing showed that tamsulosin caused a concentrated-dependent relaxation of endothelium intact renal artery rings precontracted with a high level of KCl (60 mM) or phenylephrine (PE) (10-5 M), also tamsulosin exhibited potent inhibitory effects on PE, and less potent on KCl-induced contractions. Renal artery rings preincubated with potassium (K+) channels blocker glibenclamide (GLIB), 4-aminopyridine (4-AP), prostaglandin I2 (PGI2) inhibitor (indomethacin) and epoxyeicosatrienoic acid (EET) inhibitor (Clotrimazole) have a significant effect in relaxation induced by tamsulosin. On the other side, subtype blockers from other K+ channels (tetraethylammonium, TEA), barium chloride (BaCl2) and inhibitor of nitric oxide (NO) synthase (L-Name) not exhibited any role in the relaxation effect of tamsulosin. Furthermore, the role of L-type of calcium channels (nifedipine) and tamsulosin, suggesting a Ca++ channel blocking mechanism has a relaxant effect in the urinary tract smooth muscles. Thus, from these results, it can be concluded that both potassium and calcium channels play an important role in relaxation effect of tamsulosin, which is mediated possibly through blocking of KV, KATP, PGI2, EET and voltage-dependent calcium channels.


Tamsulosin, Smooth muscles relaxation, Renal artery, Channel blockers.


[1] T. Campschroer, X. Zhu, R. W. Vernooij, and M. T. Lock, "Alpha?blockers as medical expulsive therapy for ureteral stones," Cochrane Database of Systematic Reviews, no. 4, 2018.
[2] A. C. Meltzer et al., "Effect of tamsulosin on passage of symptomatic ureteral stones: a randomized clinical trial," JAMA internal medicine, vol. 178, no. 8, pp. 1051-1057, 2018.
[3] D. Bos and A. Kapoor, "Update on medical expulsive therapy for distal ureteral stones: Beyond alpha-blockers," Canadian Urological Association Journal, vol. 8, no. 11-12, p. 442, 2014.
[4] E. El-Barky, Y. Ali, M. Sahsah, A. A. Terra, and E. O. Kehinde, "Site of impaction of ureteric calculi requiring surgical intervention," Urolithiasis, vol. 42, no. 1, pp. 67-73, 2014.
[5] O. R. Karabacakb et al., "Alpha adrenergic receptors in renal pelvis and calyces: can rat models be used?," International braz j urol, vol. 40, no. 5, pp. 683-689, 2014.
[6] M. Hennenberg et al., "Non?Adrenergic, Tamsulosin?Insensitive Smooth Muscle Contraction is Sufficient to Replace ?1?Adrenergic Tension in the Human Prostate," The Prostate, vol. 77, no. 7, pp. 697-707, 2017.
[7] M. Kaplan, "Alpha-blockers in the Treatment of Hypertension," NEFROLOGÍA, vol. XX, 2000.
[8] H. Wang, L. B. Man, G. L. Huang, G. Z. Li, and J. W. Wang, "Comparative efficacy of tamsulosin versus nifedipine for distal ureteral calculi: a meta-analysis," Drug design, development and therapy, vol. 10, p. 1257, 2016.
[9] A.-f. A.-m. Ahmed and A.-y. S. Al-sayed, "Tamsulosin versus alfuzosin in the treatment of patients with distal ureteral stones: prospective, randomized, comparative study," Korean journal of urology, vol. 51, no. 3, pp. 193-197, 2010.
[10] H. Krishnasamy, "A prospective study Research, 9,(11), 61523-61526," Key words, 2017.
[11] J. W. Lee, M. Y. Lee, and I. Y. Seo, "In-vitro study on ureteral smooth muscle contractility with tamsulosin, nifedipine, and terpene mixture (Rowatinex®)," MINERVA UROLOGICA E NEFROLOGICA, vol. 67, no. 2, pp. 91-96, 2015.
[12] M. F. Vrolijk, G. R. Haenen, A. Opperhuizen, E. H. Jansen, P. M. Schiffers, and A. Bast, "The supplement-drug interaction of quercetin with tamsulosin on vasorelaxation," European journal of pharmacology, vol. 746, pp. 132-137, 2015.
[13] L. Villa et al., "Effects by silodosin on the partially obstructed rat ureter in vivo and on human and rat isolated ureters," British journal of pharmacology, vol. 169, no. 1, pp. 230-238, 2013.
[14] O. A. Al-Habib and M. S. Shekha, "Vasorelaxant effect of aqueous extract of Crataegus azarolus aronia and quercetin on isolated albino rat's thoracic aorta," J Duhok Univ, vol. 13, pp. 1-9, 2010.
[15] I.-W. Shin et al., "Etomidate attenuates phenylephrine-induced contraction in isolated rat aorta," Canadian Journal of Anesthesia, vol. 52, no. 9, p. 927, 2005.
[16] Z. Qu et al., "Vasorelaxant effects of Cerebralcare Granule® are mediated by NO/cGMP pathway, potassium channel opening and calcium channel blockade in isolated rat thoracic aorta," Journal of ethnopharmacology, vol. 155, no. 1, pp. 572-579, 2014.
[17] A. Ahmed and I. Maulood, "The roles of potassium channels in contractile response to urotensin-II in mercury chloride induced endothelial dysfunction in rat aorta," Iranian Journal of Veterinary Research, vol. 19, no. 3, p. 208, 2018.
[18] R. Eckert, A. Karsten, J. Utz, and M. Ziegler, "Regulation of renal artery smooth muscle tone by ? 1-adrenoceptors: role of voltage-gated calcium channels and intracellular calcium stores," Urological research, vol. 28, no. 2, pp. 122-127, 2000.
[19] H. Kontani and C. Shiraoya, "Method for simultaneous recording of the prostatic contractile and urethral pressure responses in anesthetized rats and the effects of tamsulosin," The Japanese Journal of Pharmacology, vol. 90, no. 3, pp. 281-290, 2002.
[20] L.-M. Tang, J.-T. Cheng, and Y.-C. Tong, "Inhibitory effect of buflomedil on prostate ?1A adrenoceptor in the Wistar rat," Neuroscience letters, vol. 367, no. 2, pp. 224-227, 2004.
[21] A. Karadeniz, ?. Pi?kin, D. E?siz, and L. Altinta?, "Relaxation Responses of Trigonal Smooth Muscle from Rabbit by Alpha 1-Adrenoceptor Antagonists Alfuzosin, Doxazosin and Tamsulosin," Acta Veterinaria Brno, vol. 77, no. 1, pp. 81-88, 2008.
[22] S. Troxel, A. Jones, L. Magliola, and J. Benson, "Physiologic effect of nifedipine and tamsulosin on contractility of distal ureter," Journal of endourology, vol. 20, no. 8, pp. 565-568, 2006.
[23] S. Jähnichen, M. Eltze, and H. H. Pertz, "Evidence that ?1B-adrenoceptors are involved in noradrenaline-induced contractions of rat tail artery," European journal of pharmacology, vol. 488, no. 1-3, pp. 157-167, 2004.
[24] P. H. Van der Graaf, N. P. Shankley, and J. W. Black, "Analysis of the activity of ?1?adrenoceptor antagonists in rat aorta," British journal of pharmacology, vol. 118, no. 2, pp. 299-310, 1996.
[25] K. Hasunuma, D. M. Rodman, and I. F. Mcmurtry, "Effects of K+ Channel Blockers on Vascular Tone in the Perfused Rat Lung1-3," Am Rev Respir Dis, vol. 144, pp. 884-887, 1991.
[26] P. Crowley and H. Gallagher, "Clotrimazole as a pharmaceutical: past, present and future," Journal of applied microbiology, vol. 117, no. 3, pp. 611-617, 2014.
[27] P. Tep-Areenan and P. Sawasdee, "Vasorelaxant effects of 5, 7, 4?-trimethoxyflavone from Kaepmferia parviflora in the rat aorta," IJP-International Journal of Pharmacology, vol. 6, no. 4, pp. 419-424, 2010.
[28] C. Elam, M. Lape, J. Deye, J. Zultowsky, D. T. Stanton, and S. Paula, "Discovery of novel SERCA inhibitors by virtual screening of a large compound library," European journal of medicinal chemistry, vol. 46, no. 5, pp. 1512-1523, 2011.
[29] K. Davenport, A. G. Timoney, and F. X. Keeley, "A comparative in vitro study to determine the beneficial effect of calcium?channel and ?1?adrenoceptor antagonism on human ureteric activity," BJU international, vol. 98, no. 3, pp. 651-655, 2006.
[30] R. C. Young, R. Schumann, and P. Zhang, "Nifedipine block of capacitative calcium entry in cultured human uterine smooth-muscle cells," Journal of the Society for Gynecologic Investigation, vol. 8, no. 4, pp. 210-215, 2001.
[31] T. A. Manuck, "Pharmacogenomics of preterm birth prevention and treatment," BJOG: An International Journal of Obstetrics & Gynaecology, vol. 123, no. 3, pp. 368-375, 2016.
[32] S. Sigala et al., "Evidence for the presence of ?1 adrenoceptor subtypes in the human ureter," Neurourology and Urodynamics: Official Journal of the International Continence Society, vol. 24, no. 2, pp. 142-148, 2005.
[33] L. Haddad et al., "Impact of tamsulosin and nifedipine on contractility of pregnant rat ureters in vitro," The Journal of Maternal-Fetal & Neonatal Medicine, vol. 31, no. 2, pp. 191-196, 2018.
[34] V. P. Srivastava, C. P. Pandey, M. A. Raza, K. K. Agarwal, and C. Chauhan, "Effectiveness of alpha blockers+ corticosteroids in expulsion of ureteric stones: a clinical observation," International Journal of Medical Science and Public Health, vol. 3, no. 6, pp. 693-696, 2014.


How to Cite

A. M. Asaad and I. Salih Ibraheem Kakey, “The relaxant effect of Tamsulosin in the vascular reactivity of goat isolated renal artery”, KJAR, pp. 106–119, Aug. 2019, doi: 10.24017/science.2019.ICHMS.11.

Article Metrics





Pure and Applied Science