Determination the site of antibiotic resistance genes in Escherichia coli isolated From Urinary Tract Infection

https://doi.org/10.24017/science.2018.3.2

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Authors

  • Karzan Abdulmuhsin Mohammad Medical Analysis Department, Science Faculty | Ishik university, College of Education | Salahaddin University, Erbil, Iraq
  • Zirak F. Ahmed Biology Department, College of Education, Salahaddin University, Erbil, Iraq
  • Bayar A Mohammed Biology Department, College of Education, Salahaddin University, Erbil, Iraq
  • Rasti H Saeed Biology Department, College of Education, Salahaddin University, Erbil, Iraq

Abstract

This study includes isolation of 25 isolates of Escherichia coli (E. coli  ) strain from urinary tract samples in a pregnant woman. Microbiological and biochemical tests were used to identify the resistant bacteria of this genus. Screening methods were used to determine bacterial isolates for their resistance to 10 antibiotics include: Amikacin (Ak), Amoxicillin (Ax), Ampicillin (Ap), Chloramphenicol (Cm), Ciprofloxacin (Cip), Erythromycin (Er), Nalidixic acid (Nal), Penicillin (Pen), Tetracycline (Tet) and Trimethoprim (Tm). The isolates E4, E9, E16, and E17 were resistant to all antibiotics used in the current  study using the disk diffusion method. In contrast, the resistance percentage for all antibiotics ranged between 28-96%. Sites of resistance genes and hemolysin production genes were characterized by tranformation techniques in the E4 and E16. The results showed that the antibiotic resistance genes of Amikacin, Erythromycin, Tetracyclin, and Trimethoprim were located on a plasmid, whereas Amoxicillin, Ampicillin, Chloramphenicol, Ciprofloxacin, Nalidixic acid and Penicillin were located on chromosomal DNA. The results also demonstrated an inability to produce alpha or beta-hemolysin indicating that the genes which are responsible for hemolysin production were also located on chromosomal DNA. 

Keywords:

E. coli , antibiotics, resistant genes, plasmid

References

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How to Cite

[1]
K. A. Mohammad, Z. F. Ahmed, B. A. Mohammed, and R. H. Saeed, “Determination the site of antibiotic resistance genes in Escherichia coli isolated From Urinary Tract Infection”, KJAR, vol. 3, no. 2, pp. 6–12, Oct. 2018, doi: 10.24017/science.2018.3.2.

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Published

18-10-2018

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Pure and Applied Science