Assessment of Herpes Simplex Virus Type 1 and 2 by IHC in Association with CD14 Antigen in Placental Tissues from Women with Miscarriage

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Zainab A. Hamid Solaf J. Ali Ali Hattem Hussain


Among many viral causes of miscarriage, maternal infections caused by herpes simplex virus type -1 (HSV-1) and Herpes simplex virus type-2 (HSV-2) infections are important causes. CD14 was implicated in immune mechanisms, and might be involved in pregnancy loss.  The aim of this study was to detect the possible occurrence of two HSV-1 and HSV-2 infections as well as the immunological factor cd14 in placental tissues from patients with spontaneous abortion using immunohistochemistry techniques. Immunohistochemistry technique assay was used to detect placental infection with HSV-1& HSV-2 as well as CD14 in 40 women with spontaneous miscarriage and in 40 healthy deliveries in Baghdad/Iraq. The IHC- expressions of HSV-1, HSV-2, and CD14 proteins were detected in 4(10%), 15(37.5%), 15(37.5%) in placental tissues from miscarriage women, respectively. The association between CD14 with HSV-1 and HSV-2 among the placental tissues from miscarriage patients was constituting (5%), (20%) with HSV-1 & HSV-2, respectively. The considerable proportion of HSV-1, HSV-2, and infections found in the placental tissues from miscarriage women could drag the attention of obstetricians to implicate these viruses as possible targets in the etiology of spontaneous abortion case and there is possible association of CD14 with HSV-1 and HSV-2 in women with abortion.


Herpes viruses, Immunohistochemistry, Miscarriage, Pregnancy, Placenta, CD14.


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[1] A Oliver, C Overton, "Diagnosis and management of miscarriage" Practitioner, 258 (3), PP. 25-28, 2014.
[2] ZR Khameneh, H Hanifian, R Barzegari, N Sepehrvand, "Human parvovirus B19 in Iranian pregnant women: a serologic survey, Indian Journal of Pathology and Microbiology, 57 (3), pp. 442-444, 2014.
[3] Di Stefano M, Calabrò ML, Di Gangi IM, Cantatore S, Barbierato M, "In Vitro and In Vivo Human Herpesvirus 8 Infection of Placenta," PLOS ONE, 3 (12), e4073, 2008.
[4] A Festary, V Kourí, C Correa, V Denis, "Cytomegalovirus and Herpes Simplex Infections in Mothers and Newborns in a Havana Maternity Hospital," International journal of Cuba health & medicine, 17 (1), PP. 29-34, 2015.
[5] CD Truong, W Feng, Li W, "Characteristics of Epstein-Barr virus-associated gastric cancer: a study of 235 cases at a comprehensive cancer center in U.S," Journal of Experimental and Clinical Cancer Research, 28 (1):14-21, 2009.
[6] G Straface, A Selmin, V Zanardo, M De Santis, A Ercoli, G Scambia, " Herpes Simplex Virus Infection in Pregnancy," Infectious Diseases in Obstetrics and Gynecology, 27 (1), PP. 287-293, 2012.
[7] K Hideki, Z Jian Zhang, A Makrigiannakis, S Getsios, C MacCalman, J Strauss, S Parry, "Syncytiotrophoblast Is a Barrier to Maternal-Fetal Transmission of Herpes Simplex Virus," Biology of Reproduction, 67 (5), pp. 1572–1579, 2002.
[8] S Sifakis S, E Koumantakis , M Koffa, M Ergazaki , DA Spandidos , "Detection of herpes simplex virus (HSV) in aborted material using the polymerase chain reaction technique," Gynecologic and Obstetric Investigation, 45 (2), pp. 109-115, 1998.
[9] R Gupta, T Warren, A Wald, “Genital herpes,” The Lancet, 370 (9605), pp. 2127–2137, 2007.
[10] JA Robb, K Benirschke, R Barmeyer, "Intrauterine latent herpes simplex virus infection: I. Spontaneous abortion," Human Pathology, 17 (12), pp. 1196-209, 1986.
[11] G Syridou, N Spanakis, A Konstantinidou, ET Piperaki, D Kafetzis, E Patsouris, "Detection of cytomegalovirus, parvovirus B19 and herpes simplex viruses in cases of intrauterine fetal death: association with pathological findings," Journal of Medical Virology, 80 (10):1776-1782, 2008.
[12] S Matia, S Masoud, l Chamani, R Bagheri, K Kamali, "PCR Detection of Herpes Simplex Virus in Human Placenta and Aborted Materials in Patients with Spontaneous Abortion," Archives of Clinical Infectious Diseases, 6 (2), pp.17-20, 2008.
[13] S Zhang, T Regnault, P Barker, K Botting, I McMillen, C McMillan, C Roberts, JL Morrison, "Placental Adaptations in Growth Restriction, Nutrients, 7 (1), PP. 360-389, 2015.
[14] F Fabiana, O Lisiane, R Gisele, "Herpes Simplex Virus: Prevalence in Placental Tissue and Incidence in Neonatal Cord Blood Samples," Journal of Medical Virology, 6 (1), PP. 75-81, 2013.
[15] NC Kapranos , DC Kotronias, " Detection of herpes simplex virus in first trimester pregnancy loss using molecular techniques," In Vivo, 23 (5), PP. 839-842, 2009.
[16] J Gutiérrez, F Fernández, MJ Vergara, S Suárez, MJ Soto, MC Maroto, " Comparison of several ELISA tests for detecting the presence of IgG and IgM against herpes simplex viruses," Microbios, 103(405), PP. 127-32, 2000.
[17] M Makhseed, R Raghupathy, F Azizieh, A Omu, E Al-Shamali , L Ashkanani , " Th1 and Th2 cytokine profiles in recurrent aborters with successful pregnancy and with subsequent abortions," Human Reproduction, 16 (10), pp. 2219-2226, 2001.
[18] LM Weiss, YY Chen, "EBER in situ hybridization for Epstein-Barr virus," .Methods in Molecular Biology, 999 (2), pp. 223-230, 2013.
[19] A Eskild, S Jeansson, B Stray‐Pedersen, P Jenum, " Herpes simplex virus type‐2 infection in pregnancy: no risk of fetal death: results from a nested case–control study within 35,940 women," Obstetrics and Gynecology, 109 (9), pp. 1030-1035, 2003.
[20] E Kalu, C Ojide, A Chuku, I Chukwuonye, F Agwu, V Nwadike, F Korie, G Okafor, " Obstetric outcomes of human herpes virus-2 infection among pregnant women in Benin, Nigeria," Nigerian Journal of Clinical Practice, 18(4):453-461, 2015.
[21] D Ranoa, S Kelley, R Tapping, " Human lipopolysaccharide-binding protein (LBP) and CD14 independently deliver triacylated lipoproteins to Toll-like receptor 1 (TLR1) and TLR2 and enhance formation of the ternary signaling complex," Journal of Biology Chemistry, 288 (14), pp. 9729-41, 2013.
[22] S Quenby, M Bates, T Doig, J Brewster, Lewis-DI Jones, PM Johnson, G Vince, "Preimplantation endometrial leukocytes in women with recurrent miscarriage," Human Reproduction, 14(9):2386–2391, 1999.
[23] JE Lim, T Kim, NW Lee, MJ Oh, HJ Seol, JY Min, HJ Kim, " CD14 receptor gene promoter polymorphism: awakening maternal immunity during pregnancy," Journal of International Medical Research, 34 (2):176-82, 2006.
[24] A Reichard, N Cheemarla, N Bigley,"SOCS1/3 Expression Levels in HSV-1-Infected, Cytokine-Polarized and -Unpolarized Macrophages," Journal of Interferon & Cytokine Research, 35 (1), PP. 32-41, 2015.
[25] J Arii, M Uema, T Morimoto, "Entry of Herpes Simplex Virus 1 and Other Alphaherpesviruses via the Paired Immunoglobulin-Like Type 2 Receptor α," Journal of Virology, 83 (9):4520-4527, 2009.
[26] S Ellermann-Eriksen, "Macrophages and cytokines in the early defence against herpes simplex virus," Virology Journal, 2 (1), PP. 59-64, 2005.