Chemical Analysis and Growth Inhibitory Effect Of Rosemary Plant on Aspergillus niger

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Tara Faeq M. Salih Lana MA. Mohammed Karzan Omer Qader

Abstract

The use of natural fungicides instead of synthetic ones covers is a present trend in mycological disease control. The quality and food safeties could be preserved by the addition of natural antifungal agents as essential oils. The aim of the present study was to assess the antifungal activity of certain essential oil against the genus of Aspergillus The oil of the aerial parts of Rosemary plant that collected from Sulaimani city was analyzed by using gas chromatography mass spectrometry. The antifungal activity of Rosemary plant extract against Aspergillus niger was assessed by using agar dilution method. The results showed identification of 17 compounds, with 80.62% of Benzene, 1-methoxy-4- (1-propenyl), 6.85% 1,3,6-Octatriene, 3,7-dimethyl-, (E), 5.16% 1,3,6-Octatriene, 3,7- dimethyl and 2.83% D-Limonene constitute the highest percentage of essential oil. The result of investigation the antifungal effect of Rosemary plant extraction dilutions showed that the essential oil has significant inhibitory effect on growth of Aspergillus niger in 10 %, 12%, 14%, 16%, 18%, 20%, 22%, 24%, 26%, and 28% dilutions with the average inhibitory growth percentage 25.91%, 25.60%, 28.96%, 28.96%, 30.18%, 32.31%, 28.04%, 28.65%, 31.40% and 31.70% respectively. Large percentage antifungal activities of Rosemary oil are related with Benzene, 1-methoxy-4-(1-propenyl) as the main compound. In conclusion, the essential oils exhibited a potential inhibition activity against toxic fungi and many invitro and invivo studies are requested to find more about the antifungal activity of these extracted oils.

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References

[1] Y.Aeenechi, Medical Singulars and Medical Plants of Iran, Tehran University Press, 1991.
[2] G.Agrios,"Plant Pathology. 5th Edition, Elsevier Academic Press", Amsterdam, vol. 26-27, pp. 398-401, 2005.
[3] G.N.Agrios,"Losses caused by plant diseases," Plant Pathology, Elsevier, Oxford, UK. pp. 29-45, 2004.
[4] C.Booth,"The Genus Fusarium Commonwealth Mycological Institute," Kew, Surrey, pp. 237, 1971.
[5] R. Davicino, M.A. Mattar, Y.A. Casali, S. Graciela, E. Margarita, and B. Micalizzi,"Antifungal activity of plant extracts used in folk medicine in Argentina," RevistaPeruana de Biología, vol. 14, pp. 247-251, 2007.
[6] M.S. Demo, and M. Oliva,"Antimicrobial activity of medicinal plants from South America," In Watson, R.R., and V.R. Preedy (eds), pp. 152-164, 2008.
[7] A. Fisher, F. Immerand O. Tedin, "The Genetical Interpretation of Statistic of the Third Degree of Study of Quantitative Inheritance," Genetics, vol. 17, pp. 107-114, 1932.
[8] E. Frankel," Antioxidant activity of a rosemary extract and its constituents, carnosic acid, carnosol, and rosmarinic acid, in bulk oil and oil-in-water emulsion," Journal of Agricultural and Food Chemistry, Davis/Lausanne, vol. 44, pp. 131-135, 1996.
[9] AK. Genena, H. Hense, AS. Juniour, SM. Souza,"Rosemary (Rosmarinusofficinalis) a study of the composition, antioxidant and antimicrobial activities of extracts obtained with supercritical carbon dioxide," Ciênc. Tecnol. Aliment. Campinas, vol. 28, pp. 463-469, 2008.
[10] M. Mahboubi, MM. Feizabadi, M. Safara, "Antifungal activity of essential oils from Zatariamultiflora, Rosmarinusofficinalis, Lavandulastoechas, Artemisia sieberiBesser and Pelargonium graveolens against clinical isolates of Candida albicans," Pharmacognosy Mag, vol. 4, pp. 15-18, 2008.
[11] M. Moghtader, D. Afzali, "Study of the Antimicrobial Properties of the Essential Oil of Rosemary," American-Eurasian J. Agric. Environ. Sci, vol. 5, pp. 393-397, 2009.
[12] M.Moghtader, H. Salari and A. Farahmand, "Evaluation of the antifungal effects of rosemary oil and comparison with synthetic borneol and fungicide on the growth of Aspergillusflavus," Journal of Ecology and the Natural Environment, vol. 3, pp. 210-214, 2011.
[13] P.E.Nelson, T.A. Toussoun, and W.F.O. Marasas, "Fusarium Species. In: An Illustrated Manual for Identification," Pennsylvania State University Press, University Park, pp. 193, 1983.
[14] N. Okamura, H,Haraguchi, K. Hashimoto, A. Yagi,"Flavonoids in Rosmarinusofficinalis leaves"Phytochemistry, 37(5): 1463-1466, 1994.
[15] M. Oluwatuyi, G. W.Kaatz, S. Gibbons, "Antibacterial and resistance modifying activity of Rosmarinusofficinalis," Phytochemistry, London/Detroit, vol. 65, pp. 3249-3254, 2004.
[16] MM. Ozcan, JC. Chalchat, "Chemical composition and antifungal activity of rosemary (Rosmarinusofficinalis L.) oil from Turkey," International J. Food Sci. Nutr., vol.59, pp. 691-698, 2008.
[17] D. K.Pandey, N. Tripathi, R. Tripathi and S. Dixit, "Fungitoxic and Phytotoxic properties of the essential oil of H," suaveolenszeitschrift fur pflanzenkraneatenand pflanzenschutz, vol. 89, pp. 344-349, 1982.
[18] S. Prabodh, H. JoneTyler, M. Lopez Elizabeth, L. McFeetersRobert, A. Awadh AliNasser, M.Iman, G. Al-kafAli and N. Setzer William, "Chemotypic Characterization and Biological Activity of Rosmarinusofficinalis," Foods, vol. 6, pp. 20, 2017.
[19] R. Sharma, "Pathogenicity of Aspergillusniger in plants,"Cibtech Journal of Microbiology, pp. 2319-3867, 2012.
[20] KM. Soliman, R. Badeaa, "Effect of oil extracted from some medicinal plants on different mycotoxigenic fungi," Food Chem Toxicol, vol.40, pp. 1669-1675, 2002.
[21] A. Zargari, Medical Plants, 5th Edition, Tehran University Press, 1995.