Antibacterial Effect of Fixed and Volatile Oils against Gram-positive and Gram-negative Bacteria

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Tara Faiq M. Salih Sakar Ahmed Abdullah Salar Ibrahim Ali Daryan Kamal Khder


Antibiotic resistance phenomena among pathogenic bacteria considered as a major health problem ‎and associated with increased mortality or long-term hospitalization, which lead to open a new era by using ‎plant and herbal extracts as an alternative source of various chemotherapeutic drugs, also to increase antibiotic efficiency by combining with plant extract for obtaining a powerful and broad ‎spectrum action. The current investigation aims to investigate antibacterial actions of fixed oils of (Olea europaea L., ‎Ricinus communis L. and Linum usitatissimum) and volatile oil of (Nigella sativa, Curcuma longa L and ‎Zingiber officinale) against both Staphylococcus aureus strain (6734151) and Escherichia coli ‎strain (5344572). This study conducted on antibacterial effect of six ‎different extracted oils from medical herbs. The findings revealed that the oil extracts have different ‎antibacterial activities with efficacy. Bacterial inhibition zone was ‎detected by using ‎disk diffusion method. Furthermore, volatile oil of N. sativa ‎showed a great inhibitory action ‎against ‎resistant S. aureus, which was (27.7± 1.2 mm). ‎The antimicrobial effects of other fixed and volatile oils ‎against S. aureu, the inhibition zone was (10 ± 1.0 mm) for (Zingiber officinale), ‎ (9 ± 1.0 mm) for Ricinus communis L., (7.7 ‎‎‎± 0.6 mm) for Olea europea L., (7.3 ± 0.6 mm) for Linum usitatissimum and for Curcuma ‎longa L. was (6.7 ± 0.6mm). Moreover, antimicrobial effect of N. sativa against ‎ E. coli was more ‎‎active in comparison with other oils, while other oils showed a slight antibacterial effect‎. In conclusion, volatile oil of N. sativa reveals great antibacterial activities in comparison with other extracted oils. 



Black seed, antibacterial activity, fixed oil and volatile oil.


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