Treating Antibiotic Resistance Genes in Proteus Spp. were Isolated from Renal Stone Patients by Crataegus rhipidophylla and Adiantum capillus
https://doi.org/10.24017/science.2018.2.17
Abstract views: 1857 / PDF downloads: 902Abstract
Nine isolates of Proteus spp. were isolated from 100 urine samples of renal stone patients which were the urine specimens obtained directly from Sulaimani Teaching Hospital Laboratory, and identified according to the cultural characteristic, morphological, biochemical examination. The antibiotic susceptibility test for all isolates were conducted to nine antimicrobial agents including (Ciprofloxacin (Cip), Tetracycline(TE), Neomycin (N), Gentamicin (CN), Erythromycin (E), Nitrofurantoin (F), Naldixic acid (NA), Imipenem (IPM), Amoxicillin (AX). Plasmid analysis of these isolates showed presence are (22) Kb plasmid. Curing of antibiotic resistance genes by using methanol extracts for leave of Crataegus rhipidophylla and Adiantum capillus was performed. The minimum inhibitory concentration of these medicinal plants through methanol extracts which were 5000 µg/ml and 1000 µg/ml for Ailanthus altissima and Adiantum capillus respectively. The Sub minimum inhibition concentration (SMIC) was also determined. The results of transformation and curing experiments revealed that SMIC of Ailanthus altissima extract was cured or eliminated plasmid completely, and (SMIC) of Adiantum capillus was cured (CN, E, and AX) resistant genes.
Keywords:
Proteus sp., Plasmid, antibiotic resistance, transformation, curing, medicinal plants.
References
[1] A. H. Richard.; C. C. Pa,ela and D. F.Bruce ''Lippincotts illustrated Review: Microbiology'', 2nd Edition, 2007. (book style)
[2] F. Luzzaro, G. D. Brigante, M. M. Andrea, B. Pini, T. Giani and E. Mantengoli et al. ''Spread of multidrug-resistant Proteus mirabilis isolates producing an AmpC-type beta-lactamase: epidemiology and clinical management''. Int J Antimicrob Agents. ;33(4):328-33., 2009. [Medline].
https://doi.org/10.1016/j.ijantimicag.2008.09.007
[3] J.S. Lewis; M. Herrera; B. Wickes; J.E. Patterson and J. H. Jorgensen, ''First report of the emergence of CTX-M-type extended-spectrum beta-lactamases (ESBLs) as the predominant ESBL isolated in a U.S. health care system''. Antimicrob Agents Chemother. 51(11):4015-21. 2007 [Medline].
https://doi.org/10.1128/AAC.00576-07
[4] Pooja D. Gupta, Tannaz J. Birdi*' 'Development of botanicals to combat antibiotic resistance'' Contents lists available at ScienceDirect Journal of Ayurveda and Integrative Medicine journal (8) 266-275, 2017. (Review Article style
https://doi.org/10.1016/j.jaim.2017.05.004
[5] R.M Atlas, L.C Parks and A.E. Brown Laboratory manual of experimental microbiology Mosby-year Inc., U.S.A. 1995. (Book style).
[6] L. Warren and E. Jawetz. ''Medical microbiology and immunology''. Hall International. Six edd. Lange Medical Books/McGraw-Hill. Medical Publishing. New York. 2000, (book style).
[7] A.W.Bauer , Kirby WMM, Sherris JC, Turk M. "Antibiotic susceptibility testing by a standardized single disk method''. Am J Clin Pathol ;45:493-6. 1966.Medline Web of Science Google Scholar,
https://doi.org/10.1093/ajcp/45.4_ts.493
[8] Clinical and Laboratory Standards Institute. Performance standards for antimicrobial disk susceptibility tests. Approved standard M2-A10. Wayne, PA: Clinical and Laboratory Standards Institute; 2009.
[9] National Committee for Clinical Laboratory Standards Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. National Committee for Clinical Laboratory Standards: Wayne, PA. 2000.
[10] S. S.Handa, Khanuja SPS, Longo G,Rakesh DD '' Extraction Technologies for Medicinal and Aromatic Plants'', (1stedn), no. 66. Italy: United Nations Industrial Development Organization and the International Centre for Science and High Technology., 2008. (technical report style)
[11] R. W. Bussmann,. 1 G. Malca-García,2 A. Glenn,1 D. Sharon,1 G. Chait,3 D. Díaz,2 K. Pourmand,4 B. Jonat,4S. Somogy,5 G. Guardado,6 C. Aguirre,6 R. Chan,6 K. Meyer,1 A. Kuhlman,1 A. Townesmith,1 J. Effio-Carbajal,2F. Frías-Fernandez,2 and M. Benito2 , ''Minimum inhibitory concentrations of medicinal plants used in Northern Peru as antibacterialremedies'',J.Ethnopharmacol;132(1):101-108. 2010 (journal style) PMCID: PMC2956840 NIHMSID: NIHMS226166 PMID: 20678568
[12] H. C. Birnboim, and J. Doly. "A rapid alkaline extraction procedure for screening recombination plasmid DNA''. Nucleic Acid Res 7: 1513- 1524. (1979).
https://doi.org/10.1093/nar/7.6.1513
[13] T. Nancy, and T. Janine. ''Fundamental Bacterial Genetics''. by Blackwell Since Ltd a Blackwell publishing company. 2004.. 350 Main Street, Malden, MA02148- 5020. USA
[14] W.P.M Hoekstra, H.E.N. Bergmans and E.M. Zuidweg ''Tranformation in Escherichia coli. Studies on the nature of donor DNA after uptake an interaction genetic'', Camb., 35: 281-285.; 1980. (research style)
https://doi.org/10.1017/S0016672300014142
[15] A. Puhler, and N.K. Timmis. ''Advanced in molecular genetics'' springerverlag berlin Heidelberg, New York., 1984.
[16] Pei Yun Lee,1 John Costumbrado,1 Chih-Yuan Hsu,1 and Yong Hoon Kim1, ''Agarose Gel Electrophoresis for the Separation of DNA Fragments'', Journal of Visualized Experiments; (62): 3923. 2012
doi: 10.3791/3923 PMCID: PMC4846332
https://doi.org/10.3791/3923
[17] N.R. Krieg, ''Enrichment and isolation. In manual of methods for general bacteriology''. Gerhardt, P. (eds). American society for microbiology, 143-153. (1984)
[18] A.m. Sayran, ''A Bacteriological study on the incidence of urinary tract infections in rizgary teaching hospital in Erbil city''. Msc. Theses College of Science University of Salahadin. Erbil - Iraq, (1995).
[19] B. A. Adeniyi.; C.C. Amajoyi and S. I. Smith. "Plasmid profiles of multidrug resistant local uropathogenic Escherichia coli, Klebsiella spp., Proteus spp. And Pseudomonas spp. Isolates" Journal of Biological Sciences 6(3) 527-531, (2006). (journal style) ISSN 1727-3048
https://doi.org/10.3923/jbs.2006.527.531
[20] A. M. Srwa and K.K. Adel, ''Site Determination of Antibiotic Resistant Genes in E. coli Isolated from Different Sources''. Kirkuk Scientific studies journal. Volume 4 Number 3; (2009). ISSN 1992-0849
[21] Z. Zedan Ibraheim, S. Amany Ahmed, and G. Yaser Gouda, ''Phytochemical and biological studies of Adiantum capillus-''. veneris L. Saudi Pharm J., 19(2): 65-74 ;2011. doi: 10.1016/j.jsps.2011.01.007
https://doi.org/10.1016/j.jsps.2011.01.007
[22] Muhammad O. Muhammad, Srwa A. Muhammad and Ibrahim S. Hamad, ''Bacteriological and Molecular Genetic Study of Salmonella typhi Isolated from Patient with Enteric Fever'', JOURNAL OF KOYA UNIVERSITY, 2009. ISSN 2073- 0713 No: 12
[23] A. Smith-Palmer, J. Stewart and L. Ftfe. ''Antimicrobial propertiesof plant essential oils and essences against five importantfood- borne pathogens in food Microbiology'', 26: 118-112, 1998.
https://doi.org/10.1046/j.1472-765X.1998.00303.x
[24] K.H. Khan, ''Role of Emblica officinalis in medicine'', a review Botany Research International, 2, 218-228, 2009.
[25] M. Krishnaveni, and S. Mirunalini, ''Therapeutic potential of Phyllanthus emblica (amla) the ayurvedic wonder''. Journal of basic and clinical physiology and pharmacology, 21, 93-105, 2010.
https://doi.org/10.1515/JBCPP.2010.21.1.93
[2] F. Luzzaro, G. D. Brigante, M. M. Andrea, B. Pini, T. Giani and E. Mantengoli et al. ''Spread of multidrug-resistant Proteus mirabilis isolates producing an AmpC-type beta-lactamase: epidemiology and clinical management''. Int J Antimicrob Agents. ;33(4):328-33., 2009. [Medline].
https://doi.org/10.1016/j.ijantimicag.2008.09.007
[3] J.S. Lewis; M. Herrera; B. Wickes; J.E. Patterson and J. H. Jorgensen, ''First report of the emergence of CTX-M-type extended-spectrum beta-lactamases (ESBLs) as the predominant ESBL isolated in a U.S. health care system''. Antimicrob Agents Chemother. 51(11):4015-21. 2007 [Medline].
https://doi.org/10.1128/AAC.00576-07
[4] Pooja D. Gupta, Tannaz J. Birdi*' 'Development of botanicals to combat antibiotic resistance'' Contents lists available at ScienceDirect Journal of Ayurveda and Integrative Medicine journal (8) 266-275, 2017. (Review Article style
https://doi.org/10.1016/j.jaim.2017.05.004
[5] R.M Atlas, L.C Parks and A.E. Brown Laboratory manual of experimental microbiology Mosby-year Inc., U.S.A. 1995. (Book style).
[6] L. Warren and E. Jawetz. ''Medical microbiology and immunology''. Hall International. Six edd. Lange Medical Books/McGraw-Hill. Medical Publishing. New York. 2000, (book style).
[7] A.W.Bauer , Kirby WMM, Sherris JC, Turk M. "Antibiotic susceptibility testing by a standardized single disk method''. Am J Clin Pathol ;45:493-6. 1966.Medline Web of Science Google Scholar,
https://doi.org/10.1093/ajcp/45.4_ts.493
[8] Clinical and Laboratory Standards Institute. Performance standards for antimicrobial disk susceptibility tests. Approved standard M2-A10. Wayne, PA: Clinical and Laboratory Standards Institute; 2009.
[9] National Committee for Clinical Laboratory Standards Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. National Committee for Clinical Laboratory Standards: Wayne, PA. 2000.
[10] S. S.Handa, Khanuja SPS, Longo G,Rakesh DD '' Extraction Technologies for Medicinal and Aromatic Plants'', (1stedn), no. 66. Italy: United Nations Industrial Development Organization and the International Centre for Science and High Technology., 2008. (technical report style)
[11] R. W. Bussmann,. 1 G. Malca-García,2 A. Glenn,1 D. Sharon,1 G. Chait,3 D. Díaz,2 K. Pourmand,4 B. Jonat,4S. Somogy,5 G. Guardado,6 C. Aguirre,6 R. Chan,6 K. Meyer,1 A. Kuhlman,1 A. Townesmith,1 J. Effio-Carbajal,2F. Frías-Fernandez,2 and M. Benito2 , ''Minimum inhibitory concentrations of medicinal plants used in Northern Peru as antibacterialremedies'',J.Ethnopharmacol;132(1):101-108. 2010 (journal style) PMCID: PMC2956840 NIHMSID: NIHMS226166 PMID: 20678568
[12] H. C. Birnboim, and J. Doly. "A rapid alkaline extraction procedure for screening recombination plasmid DNA''. Nucleic Acid Res 7: 1513- 1524. (1979).
https://doi.org/10.1093/nar/7.6.1513
[13] T. Nancy, and T. Janine. ''Fundamental Bacterial Genetics''. by Blackwell Since Ltd a Blackwell publishing company. 2004.. 350 Main Street, Malden, MA02148- 5020. USA
[14] W.P.M Hoekstra, H.E.N. Bergmans and E.M. Zuidweg ''Tranformation in Escherichia coli. Studies on the nature of donor DNA after uptake an interaction genetic'', Camb., 35: 281-285.; 1980. (research style)
https://doi.org/10.1017/S0016672300014142
[15] A. Puhler, and N.K. Timmis. ''Advanced in molecular genetics'' springerverlag berlin Heidelberg, New York., 1984.
[16] Pei Yun Lee,1 John Costumbrado,1 Chih-Yuan Hsu,1 and Yong Hoon Kim1, ''Agarose Gel Electrophoresis for the Separation of DNA Fragments'', Journal of Visualized Experiments; (62): 3923. 2012
doi: 10.3791/3923 PMCID: PMC4846332
https://doi.org/10.3791/3923
[17] N.R. Krieg, ''Enrichment and isolation. In manual of methods for general bacteriology''. Gerhardt, P. (eds). American society for microbiology, 143-153. (1984)
[18] A.m. Sayran, ''A Bacteriological study on the incidence of urinary tract infections in rizgary teaching hospital in Erbil city''. Msc. Theses College of Science University of Salahadin. Erbil - Iraq, (1995).
[19] B. A. Adeniyi.; C.C. Amajoyi and S. I. Smith. "Plasmid profiles of multidrug resistant local uropathogenic Escherichia coli, Klebsiella spp., Proteus spp. And Pseudomonas spp. Isolates" Journal of Biological Sciences 6(3) 527-531, (2006). (journal style) ISSN 1727-3048
https://doi.org/10.3923/jbs.2006.527.531
[20] A. M. Srwa and K.K. Adel, ''Site Determination of Antibiotic Resistant Genes in E. coli Isolated from Different Sources''. Kirkuk Scientific studies journal. Volume 4 Number 3; (2009). ISSN 1992-0849
[21] Z. Zedan Ibraheim, S. Amany Ahmed, and G. Yaser Gouda, ''Phytochemical and biological studies of Adiantum capillus-''. veneris L. Saudi Pharm J., 19(2): 65-74 ;2011. doi: 10.1016/j.jsps.2011.01.007
https://doi.org/10.1016/j.jsps.2011.01.007
[22] Muhammad O. Muhammad, Srwa A. Muhammad and Ibrahim S. Hamad, ''Bacteriological and Molecular Genetic Study of Salmonella typhi Isolated from Patient with Enteric Fever'', JOURNAL OF KOYA UNIVERSITY, 2009. ISSN 2073- 0713 No: 12
[23] A. Smith-Palmer, J. Stewart and L. Ftfe. ''Antimicrobial propertiesof plant essential oils and essences against five importantfood- borne pathogens in food Microbiology'', 26: 118-112, 1998.
https://doi.org/10.1046/j.1472-765X.1998.00303.x
[24] K.H. Khan, ''Role of Emblica officinalis in medicine'', a review Botany Research International, 2, 218-228, 2009.
[25] M. Krishnaveni, and S. Mirunalini, ''Therapeutic potential of Phyllanthus emblica (amla) the ayurvedic wonder''. Journal of basic and clinical physiology and pharmacology, 21, 93-105, 2010.
https://doi.org/10.1515/JBCPP.2010.21.1.93
Downloads
How to Cite
[1]
S. Ali Mohammed, M. Abdul Aziz Hama Ali, and D. Lattif Mohammed, “Treating Antibiotic Resistance Genes in Proteus Spp. were Isolated from Renal Stone Patients by Crataegus rhipidophylla and Adiantum capillus”, KJAR, vol. 3, no. 2, pp. 102–106, Jul. 2018, doi: 10.24017/science.2018.2.17.
Article Metrics
Published
23-07-2018
Issue
Section
Pure and Applied Science
